abstract：:Neuraminidase was produced by 32.1% and 28.5% of Porphyromonas from dogs with and without periodontitis, respectively; and by 31.8% of bacteria from humans. The presence of neuraminidase in Porphyromonas spp. suggests that this enzyme can be involved with the pathogenesis of the periodontal disease, and the use of thi...

journal_title：Journal of microbiological methods

authors： de Assis PR,Nakano V,Senhorinho GN,Avila-Campos MJ

更新日期：2013-09-01 00:00:00

abstract：:An improved approach for simultaneous detection of Cryptosporidium parvum and Giardia lamblia (oo)cysts in soil is described. Recoveries>70% were obtained for concentrations>55 and 21 (oo)cysts g(-1) for C. parvum and G. lamblia, respectively. The limits of detection were determined to be<5 (oo)cysts g(-1) soil. ...

journal_title：Journal of microbiological methods

authors： Orlofsky E,Gillor O,Melli A,Miller W,Wuertz S,Bernstein N,Shapiro K

更新日期：2013-09-01 00:00:00

abstract：:The plant root interface is a hot spot for microbial activities. Root exudates are the key compounds that drive microbial performance. However quality and amount of root exudates are highly dynamic in time and space, thus a direct influence of a single compound on a microbial community composition is fairly impossible...

journal_title：Journal of microbiological methods

authors： Ziegler M,Engel M,Welzl G,Schloter M

更新日期：2013-07-01 00:00:00

abstract：:In this study the influence of X-ray computed tomography (XRCT) on the microbial community structure and function in soils has been investigated. Our results clearly indicate that XRCT of soil samples has a strong impact on microbial communities and changes structure and function significantly due to the death of sele...

journal_title：Journal of microbiological methods

authors： Fischer D,Pagenkemper S,Nellesen J,Peth S,Horn R,Schloter M

更新日期：2013-05-01 00:00:00

abstract：:Fourier-transform infrared (FTIR) spectroscopy was evaluated as an alternative method for serotyping encapsulated Streptococcus pneumonia strains. Sixty-nine invasive strains from the Microbiology Laboratory of the Medicine Faculty of University of Lisbon were selected for the analysis. The encapsulated strains used i...

journal_title：Journal of microbiological methods

authors： Vaz M,Meirinhos-Soares L,Sousa CC,Ramirez M,Melo-Cristino J,Lopes JA

更新日期：2013-05-01 00:00:00

abstract：:A standard material comprising a few viable cells of a microorganism is essential for rational validation of microbiological methods. We propose a method of a flow cytometric sorting of cells stained with 6-carboxyfluorescein diacetate. The feasibility of tryptic soy medium in this method is demonstrated with 5 strain...

journal_title：Journal of microbiological methods

authors： Matsuoka H,Shigetomi T,Funabashi H,Saito M,Igimi S

更新日期：2013-04-01 00:00:00

abstract：:Viability PCR (v-PCR) as a method to selectively detect intact live cells has gained considerable interest over the last years with an increasing number of applications. The principle is based on treatment of microbiological samples with a viability dye prior to extraction of genomic DNA and its amplification. The dye...

journal_title：Journal of microbiological methods

authors： Nkuipou-Kenfack E,Engel H,Fakih S,Nocker A

更新日期：2013-04-01 00:00:00

abstract：:In this study extruded bean was used as a nitrogen source substitute in culture medium formulation. A 3-factor simplex-lattice mixture design was used to establish better growth conditions. Completely substituted medium resulted in 43% of increase in the growth of Saccharomyces cerevisiae. Mixtures containing 1% extru...

journal_title：Journal of microbiological methods

authors： Batista KA,Bataus LA,Campos IT,Fernandes KF

更新日期：2013-03-01 00:00:00

abstract：:Antibiotic treatments can lead to a disruption of the human microbiota. In this in-vitro study, the impact of antibiotics on adult intestinal microbiota was monitored in a new high-throughput approach: a fermentation screening-platform was coupled with a phylogenetic microarray analysis (Intestinal-chip). Fecal inocul...

journal_title：Journal of microbiological methods

authors： Ladirat SE,Schols HA,Nauta A,Schoterman MH,Keijser BJ,Montijn RC,Gruppen H,Schuren FH

更新日期：2013-03-01 00:00:00

abstract：:Despite its long-standing history as a model organism, Neurospora crassa has limited tools for repetitive gene deletions utilizing recyclable self-excising marker systems. Here we describe, for the first time, the functionality of a bacterial recombination system employing β-recombinase acting on six recognition seque...

journal_title：Journal of microbiological methods

authors： Szewczyk E,Kasuga T,Fan Z

更新日期：2013-03-01 00:00:00

abstract：:There have been many recent reviews published on MALDI-TOF MS (matrix assisted laser desorption/ionization time-of-flight) MS (mass spectrometry) for identification of bacteria particularly with relevance to clinical microbiology. MALDI-TOF MS is now a mature technique for bacterial identification with great promise. ...

journal_title：Journal of microbiological methods

authors： Intelicato-Young J,Fox A

更新日期：2013-03-01 00:00:00

abstract：:Microbial adhesion is a critical step for infection and colonization of the host. Trichomonas vaginalis, a human urogenital extracellular parasite, relies on host cell adhesion for infection and pathogenesis. Although host cell adhesion of T. vaginalis is strain-dependent and it may be influenced by many environmental...

journal_title：Journal of microbiological methods

authors： Brooks AE,Parsamand T,Kelly RW,Simoes-Barbosa A

更新日期：2013-01-01 00:00:00

abstract：:The Publisher regrets that this article is an accidental duplication of an article that has already been published, http://dx.org/doi:10.1016/j.mimet.2012.12.009. The duplicate article has therefore been withdrawn. ...

journal_title：Journal of microbiological methods

authors： Pires NM,Dong T

更新日期：2012-12-20 00:00:00

abstract：:In recent years the global rise in antibiotic resistance and environmental consciousness lead to a renewed fervour to find and develop novel antibiotics, including antifungals. However, the influence of the environment on antifungal activity is often disregarded and many in vitro assays may cause the activity of certa...

journal_title：Journal of microbiological methods

authors： Troskie AM,Vlok NM,Rautenbach M

更新日期：2012-12-01 00:00:00

abstract：:Flow cytometry (FCM) is efficient in detecting both abundance and optical physiological parameters including cell size and cellular carbon content-side scatter (SSC), carotenoids-green and orange fluorescence (FL1 and FL2), and red fluorescence-chlorophylls (FL3) can be obtained by FCM. The utilization of these physio...

journal_title：Journal of microbiological methods

authors： Zhang F,Ma Y,Lin L,He J

更新日期：2012-12-01 00:00:00

abstract：:Two commercially available all-in-one swab rapid detection systems for Listeria spp. (InSite Listeria Test and Path-Chek hygiene Listeria) were tested for performance in cheese production environments and salmon processing facilities. Sampling was conducted both on clean surfaces and during production. A total of 338 ...

journal_title：Journal of microbiological methods

authors： Schirmer BC,Langsrud S,Møretrø T,Hagtvedt T,Heir E

更新日期：2012-11-01 00:00:00

abstract：:Here we demonstrate a cost effective and scalable microbial ecology sequencing platform using the Ion Torrent Personal Genome Machine (PGM). We assessed both PCR amplified 16S rRNA and shotgun metagenomic approaches and generated 100,000+ to 1,000,000+ reads using 'post-light' based sequencing technology within differ...

journal_title：Journal of microbiological methods

authors： Whiteley AS,Jenkins S,Waite I,Kresoje N,Payne H,Mullan B,Allcock R,O'Donnell A

更新日期：2012-10-01 00:00:00

abstract：:Permanent staining of faecal smears by Wheatley's trichrome technique has been used by many scientists for the detection of parasites in the past and it was found to be highly sensitive. This study was conducted to evaluate the use of Wintergreen oil in comparison with xylene in Wheatley's trichrome staining technique...

journal_title：Journal of microbiological methods

authors： Salleh FM,Anuar TS,Yasin AM,Moktar N

更新日期：2012-10-01 00:00:00

abstract：:The purpose of this study was to explore the potential of using cationic polyethylenimine (PEI) to deliver green fluorescent protein (GFP) to protozoan parasite Toxoplasma gondii. PEI/DNA polyplexes were formed using branched PEI and pEGFP-N1 plasmid with various N/P ratios that ranged from 5 to 50. With the increment...

journal_title：Journal of microbiological methods

authors： Salehi N,Peng CA

更新日期：2012-10-01 00:00:00

abstract：:Rothia dentocariosa is an indigenous microbial flora of dental plaque. To examine the bacterium population in oral cavities, a novel selective medium, designated RDSM, was developed for the isolation of R. dentocariosa. RDSM was prepared by adding 0.5 μg/ml of lincomycin, 10 μg/ml of colistin, and 40% sucrose to Heart...

journal_title：Journal of microbiological methods

authors： Uchibori S,Tsudukibashi O,Goto H,Kobayashi T,Aida M

更新日期：2012-10-01 00:00:00

abstract：:This paper reports a novel electrochemical immunosensor for the sensitive detection of staphylococcal enterotoxin A (SEA) based on self-assembly monolayer (SAM) and protein A immobilization on gold electrode. Three different methods of protein A immobilization were tested: physical adsorption, cross-linking using glut...

journal_title：Journal of microbiological methods

authors： Pimenta-Martins MG,Furtado RF,Heneine LG,Dias RS,Borges Mde F,Alves CR

更新日期：2012-10-01 00:00:00

abstract：:A novel fluorescent microbead immunoassay (FMIA) using the recombinant polypeptide SpaA415 was developed for detection of anti-Erysipelothrix spp. IgG in pig sera. The diagnostic performance of the FMIA was evaluated on samples from pigs with known and unknown Erysipelothrix spp. exposure and compared to an in-house e...

journal_title：Journal of microbiological methods

authors： Giménez-Lirola LG,Xiao CT,Halbur PG,Opriessnig T

更新日期：2012-10-01 00:00:00

abstract：:Centrifugation is a necessary concentrating step for the detection of Mycobacterium tuberculosis in a liquid culture. However, centrifugation is biologically hazardous and presents an obstacle in the development of an automated culture system. A bead-based bacterial concentration method, TRICORE, was recently develope...

journal_title：Journal of microbiological methods

authors： Mitarai S,Karinaga R,Yamada H,Mizuno K,Chikamatsu K,Aono A,Sugamoto T,Hatano T

更新日期：2012-09-01 00:00:00

abstract：:Staphylococcus aureus is a Gram-positive coccus and one of the major causes of community-acquired and hospital-acquired infections. We established the convenient and reliable experimental system for analyzing the essentiality and function of genes, the plasmid integration (PI) method. This method is based on plasmid i...

journal_title：Journal of microbiological methods

authors： Yamachika S,Onodera Y,Hiramatsu K,Takase H

更新日期：2012-09-01 00:00:00

abstract：:Streptococcus agalactiae, group B streptococci (GBS) is the leading cause of severe bacterial infections in newborns. GBS expression studies allowed the identification and characterization of virulence factors and a better understanding of the host-pathogen-environment interactions. The measurement of transcript level...

journal_title：Journal of microbiological methods

authors： Florindo C,Ferreira R,Borges V,Spellerberg B,Gomes JP,Borrego MJ

更新日期：2012-09-01 00:00:00

abstract：:A new method was established for fine visualization of bacterial subcelluar filamentous structures by freezing the bacterial cells to displace cytoplasmic matrix granules to the periphery. This method was successfully applied in immunoelectron microscopy and electron microscopic tomography, and should be applicable fo...

journal_title：Journal of microbiological methods

authors： Nakano T,Aoki H,Wu H,Fujioka Y,Nakazawa E,Sano K

更新日期：2012-07-01 00:00:00

abstract：:A common method for visualizing bacterial biofilms is through confocal laser scanning microscopy images. Current software packages separate connected-biofilm bacteria from unconnected bacteria, such as planktonic or dispersed bacteria, but do not save both image sequences, making interpretation of the two bacterial po...

journal_title：Journal of microbiological methods

authors： Sommerfeld Ross S,Reinhardt JM,Fiegel J

更新日期：2012-07-01 00:00:00

abstract：:Enterococcus faecalis, a gram-positive opportunistic pathogen, has become one of the leading causes of nosocomial infections. Normally a resident of the gastrointestinal tract, extensive use of antibiotics has resulted in the rise of E. faecalis strains that are resistant to multiple antibiotics. This, compounded with...

journal_title：Journal of microbiological methods

authors： Debroy S,van der Hoeven R,Singh KV,Gao P,Harvey BR,Murray BE,Garsin DA

更新日期：2012-07-01 00:00:00

abstract：:There is a paucity of knowledge on gene expression in dead bacterial cells. Why would this knowledge be useful? The cells are dead. However, the time duration of gene expression following cell death is often unknown, and possibly in the order of minutes. In addition, it is a challenge to determine if bacterial cells a...

journal_title：Journal of microbiological methods

authors： Trevors JT

更新日期：2012-07-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic pathogen responsible for numerous infections acquired in hospital especially in persons whose immune systems are weakened, such as with patient suffering from AIDS or cystic fibrosis. This bacterium produces a great diversity of virulence factors among them hydrogen cyanide (...

journal_title：Journal of microbiological methods

authors： Blier AS,Vieillard J,Gerault E,Dagorn A,Varacavoudin T,Le Derf F,Orange N,Feuilloley M,Lesouhaitier O

更新日期：2012-07-01 00:00:00

abstract：:We evaluated the clinical performance of the Bio-Rad Dx CT/NG/MG assay for the detection of Chlamydia trachomatis, Mycoplasma genitalium and Neisseria gonorrhoeae in urogenital samples in comparison with the Roche COBAS® TaqMan® CT assay for C. trachomatis and an in-house TaqMan PCR assay for M. genitalium. Swab speci...

journal_title：Journal of microbiological methods

authors： Le Roy C,Le Hen I,Clerc M,Arfel V,Normandin F,Bébéar C,de Barbeyrac B

更新日期：2012-06-01 00:00:00

abstract：:The purpose of this study was to determine the interactions between arsenic (As) resistant bacteria and As, using surface-enhanced Raman scattering (SERS) and Fourier transform infrared (FTIR) spectroscopy. According to our 16S rDNA results, eight bacteria isolated from the environment can be identified to four genera...

journal_title：Journal of microbiological methods

authors： Tian H,Zhuang G,Ma A,Jing C

更新日期：2012-06-01 00:00:00

abstract：:Direct extraction of Cryptosporidium DNA from 46 stools by bead-beating, guanidine thiocyanate and silica purification provided slightly lower PCR positivity (93.5% vs. 100%) and higher threshold cycle values (mean 34.93 vs. 28.03; P=0.00) than spin-column extraction from boiled, semi-purified oocyst suspensions. Howe...

journal_title：Journal of microbiological methods

authors： Elwin K,Robinson G,Hadfield SJ,Fairclough HV,Iturriza-Gómara M,Chalmers RM

更新日期：2012-04-01 00:00:00

abstract：:We developed and evaluated RNA aptamers to analyze their potential for use in detecting Salmonella Enteritidis. The selected aptamer was observed to specifically bind to Salmonella Enteritidis without any cross-reactivity to other Salmonella serovars. Thus, this study suggests that aptamers specific to Salmonella Ente...

journal_title：Journal of microbiological methods

authors： Hyeon JY,Chon JW,Choi IS,Park C,Kim DE,Seo KH

更新日期：2012-04-01 00:00:00

abstract：:Listeria monocytogenes is an important foodborne pathogen responsible for non-invasive and invasive diseases in the elderly, pregnant women, neonates and immunocompromised populations. This bacterium has many similarities with other non-pathogenic Listeria species which makes its detection from food and environmental ...

journal_title：Journal of microbiological methods

authors： Jadhav S,Bhave M,Palombo EA

更新日期：2012-03-01 00:00:00

abstract：:A PMA (propidium monoazide) pretreatment protocol, in which PMA is applied directly to membrane filters, was developed for the PCR-based quantification (PMA-qPCR) of viable Legionella pneumophila. Using this method, the amplification of DNA from membrane-damaged L. pneumophila was strongly inhibited for samples contai...

journal_title：Journal of microbiological methods

authors： Slimani S,Robyns A,Jarraud S,Molmeret M,Dusserre E,Mazure C,Facon JP,Lina G,Etienne J,Ginevra C

更新日期：2012-02-01 00:00:00

abstract：:Recent changes in Brazilian legislation for commercial disinfectants have been published due to the recent epidemic of nosocomial infections caused by rapidly growing mycobacteria (RGM) in many states of Brazil over the last 8years. One of these documents requires that all the manufacturers provide evidence of efficac...

journal_title：Journal of microbiological methods

authors： Sabagh BP,Souto Ada S,Reis LM,Silva SA,Pereira DC,Neves Mde C,Pinheiro RR,Duarte RS,Miyazaki NH,Bôas MH

更新日期：2012-02-01 00:00:00

abstract：:In many bacteria, high levels of the ubiquitous second messenger c-di-GMP have been demonstrated to suppress motility and to promote the establishment of surface-adherent biofilm communities. While molecular mechanisms underlying the synthesis and degradation of c-di-GMP have been comprehensively characterized, little...

journal_title：Journal of microbiological methods

authors： Düvel J,Bertinetti D,Möller S,Schwede F,Morr M,Wissing J,Radamm L,Zimmermann B,Genieser HG,Jänsch L,Herberg FW,Häussler S

更新日期：2012-02-01 00:00:00

abstract：:Race 3, biovar 2 strains of Ralstonia solanacearum are quarantined pathogens in Europe and Canada and Select Agent pathogens in the United States. The biovar classification of R. solanacearum strains is based on their biochemical abilities to utilize a carbohydrate panel. The standard biovar test uses bromothymol blue...

journal_title：Journal of microbiological methods

authors： Huang Q,Yan X,Wang JF

更新日期：2012-02-01 00:00:00

abstract：:In order to study functional gene expression in Streptomyces coelicolor, a mini-transposon encoding the apramycin resistance gene aac(3)IV within its inverted repeat (IR) boundaries was constructed based on IS204, which was previously identified in the genome of Nocardia asteroides YP21. The mini-transposon and IS204 ...

journal_title：Journal of microbiological methods

authors： Zhang X,Bao Y,Shi X,Ou X,Zhou P,Ding X

更新日期：2012-01-01 00:00:00